Ben Stiller
Postać: Alex (voice)
Jada Pinkett Smith
Postać: Gloria (voice)
David Schwimmer
Postać: Melman (voice)
Chris Rock
Postać: Marty / Additional Zebras (voice)
Cedric the Entertainer
Postać: Maurice (voice)
Andy Richter
Postać: Mort (voice)
Bernie Mac
Postać: Zuba (voice)
Alec Baldwin
Postać: Makunga (voice)
Sacha Baron Cohen
Postać: Julien (voice)
Sherri Shepherd
Postać: Mom (voice)
Will.i.am
Postać: Moto Moto (voice)
Elisa Gabrielli
Postać: Nana (voice)
Tom McGrath
Postać: Skipper / Lemur (voice)
Chris Miller
Postać: Kowalski (voice)
Christopher Knights
Postać: Private (voice)
Conrad Vernon
Postać: Mason (voice)
Quinn Dempsey Stiller
Postać: Baby Alex (voice)
Declan Swift
Postać: Baby Alex (voice)
Fred Tatasciore
Postać: Teetsi / Poacher #1 / Elephant (voice)
Eric Darnell
Postać: Joe the Witch Doctor / Poacher #2 (voice)
Willow Smith
Postać: Baby Glorida (voice)
Thomas Stanley
Postać: Baby Marty (voice)
Zachary Gordon
Postać: Baby Melman (voice)
Meredith Vieira
Postać: Newscaster (voice)
Lesley Stahl
Postać: Newscaster (voice)
Al Roker
Postać: Newscaster (voice)
David Soren
Postać: Lemur (voice)
Phil LaMarr
Postać: Guide (voice)
Stephen Kearin
Postać: Stephen the Giraffe / Tourist with Video Camera / Rhino (voice)
Dan O'Connor
Postać: Tourist with University Shirt / Cape Buffalo (voice)
Edie Mirman
Postać: Telephone Recording (voice)
Fergie
Postać: Hippo Girlfriend (voice) (as Stacy Ferguson)
Harland Williams
Postać: Additional Giraffe (voice)
Danny Jacobs
Postać: Tourist with New York T-Shirt (voice)
Bridget Hoffman
Postać: Female Tourist (voice)
Terrence Hardy Jr.
Postać: Cub (voice) (as Terence Hardy)
Conner Rayburn
Postać: Little Giraffe (voice)
Holly Dorff
Postać: Fish (voice)
David P. Smith
Postać: Bobby the Dik Dik (voice) (as David Smith)
Lynnanne Zager
Postać: Lioness (voice)
Jackie Gonneau
Postać: Additional Dik Dik (voice)
John Eric Bentley
Postać: Additional Dialogue (voice) (as John Bentley)
